SolRgene

Allele mining studies

How to get to R genes. 1 map-based cloning

Map-based cloning is a classic and thorough method to isolate resistance (R) genes in potato. To generate the therefore required segregating populations, resistant and susceptible plants were crossed. Populations were phenotyped for resistance, and data were included in SolRgene. Suitable populations for map-based cloning show clear segregation between resistance and susceptibility in the F1, the co-called black & white segregation. Several of such segregating populations have entered a pipe-line of R gene isolation in our laboratory; and the first R genes to P. infestans (Rpi) from this resource have recently been cloned, such as Rpi-vnt1 [4] and Rpi-sto1 [5].

How to get to R genes. 2 allele-mining

Allele-mining is a modern-day high-throughput strategy to isolate genetic variation for R gene homologues, among which functional R genes can be detected. Strongly supported with rapid growing sequence information on R genes in the potato {Visser, 2009 #3163} {van Os, 2006 #2524} [links to Potato sequencing and genomic resources, including the PGRC (Potato Genome Sequencing Consortium), Ultra dense recombination map of potato, PoMaMo, Center for Biosystems Genomics] and tomato genome [ref**], efficient allele-mining for R gene homologues (RGH) is depending on availability of phenotyped genetic material. Mining for RGH of the late blight resistance gene Rpi-blb1 from S. bulbocastanum (blb) on the SolRgene in vitro collection quickly led to identification of Rpi-blb1 homologues Rpi-sto1 and Rpi-pta1 in S. stoloniferum [13, 14]. Also other R genes acting against P. infestans (Rpi) were successfully mined on Solanum sect Petota, a.o. Rpi-vnt1, and functional R genes were identified [9, 15, 16] (Ref Figure 1, Rpi-vnt1 mining). Allele-mining data are included in SolRgene and can be linked to relevant phenotyping data.